![]() These Radiolabeled nucleotides are typically incorporated enzymatically into specific nucleic acid sequences for detection and analysis. The fragment still has the polymerase and 3'-5' exonuclease activity, but lacks the 5'-3' exonuclease activity of the holoenzyme (1). Probe labeling process radioactively label the DNA & RNA fragments for detection or purification. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED). Radiolabeling of DNA with the Klenow fragment of DNA polymerase The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. radiolabeling DNA restriction endonuclease fragments to high specific activity. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. Klenow fragment with the adsorbed DNA to GO. ![]() As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to. DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous. This method is particularly suitable for labeling restriction fragments to. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. A technique for conveniently radiolabeling DNA restriction endonuclease fragments.
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